NCT03225950

Interaction Between Immune Cells and Bacteria Associated With Periodontitis

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 90 (actual)
• Sponsor: University of Copenhagen · Academic / Other
• Sex: All
• Age: 19 Years – 60 Years
• Healthy volunteers: Accepted

Summary

This study evaluates the interaction between host immune cells and bacteria associated with periodontitis. It comprises biological material from donors with and without periodontal disease. Specifically, we collect a spit and blood sample to conduct in vitro stimulations and measurements of selected parameters related to periodontitis to clarify obscure areas in the immunologic pathogenesis of this disease.

Detailed description

Periodontitis is a prevalent, multifactorial inflammatory disease characterized by the interaction between microorganisms organized in biofilms on tooth surfaces and host immune cells, leading to an inflammatory destruction of the tooth-supporting tissues and - if left untreated - eventually tooth loss. Periodontitis affects up to 50% of the population in the United States of America, and is classified in an aggressive and a chronic form depending on genetic factors, age of onset, speed and severity of attachment loss. The onset of periodontitis is caused by an immunologic imbalance between host immune cells and residing microorganisms in subgingival pockets. The host immune cells are capable of enhancing both a protective and a destructive inflammatory response towards the microorganisms through the release of inflammatory mediators e.i. proinflammatory and antiinflammatory cytokines. The role of antibodies in periodontitis is also unclear. Some studies show an excessive antibody level against bacteria associated with periodontitis e.g. Porphyromonas gingivalis (P.g.). In general, this study contributes to a profound understanding of the host immune cells role in the onset and pathogenesis of periodontitis by comparing healthy versus diseased donors immunologic responses toward pathogene and apathogene microorganisms and their genetic background.

Conditions

• Periodontal Diseases
• Periodontitis
• Aggressive Periodontitis
• Immunologic Disease
• Microbial Disease
• Periodontal Pocket
• Inflammation
• Inflammation Gum
• Dysbiosis
• Rheumatoid Arthritis
• Generalized Aggressive Periodontitis
• Generalized Chronic Periodontitis
• Chronic Periodontitis

Interventions

Type	Name	Description
OTHER	In vitro stimulation of blood with periodontitis-associated- and control bacteria	Peripheral mononuclear blood cells are stimulated with periodontitis-associated- and control bacteria to measure the amount of positive cytokine-producing cells.
DIAGNOSTIC_TEST	Anti-CCP- and anti-P.g.-antibodies titers	Anitbody titers will be measured in saliva and serum samples.
GENETIC	Analysis of selected single nucleotide polymorphisms (SNPs)	DNA obtained from saliva samples will be used to determine the genotype of the participants for selected SNPs.
DIAGNOSTIC_TEST	periodontitis-associated bacteria presence	Determination of the presence of periodontitis-associated bacteria e.i. Porphyromonas gingivalis in saliva and blood samples.

Timeline

• Start date: 2017-02-01
• Primary completion: 2018-10-12
• Completion: 2020-03-23
• First posted: 2017-07-21
• Last updated: 2020-03-24

Locations

2 sites across 1 country: Denmark