NCT05435131

Evaluation of Biomarkers Levels in Gingival Crevicular Fluid, Saliva and Serum for Different Periodontal Diseases

Evaluation of Interleukin (IL)-38, IL-36-gamma, IL-17 and Matrix Metalloproteinase-9 Levels in Gingival Crevicular Fluid, Saliva and Serum for Periodontal Health, Gingivitis and Periodontitis

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 90 (actual)
• Sponsor: Akdeniz University · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

The aim of this study is; detection of interleukin(IL)-38, IL-36 gamma(γ) , IL-17 and matrix metalloproteinase(MMP)-9 levels in gingival crevicular fluid (GCF), saliva and serum samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples were obtained from 90 systemically healthy non-smoker individuals with periodontitis (P, n=30), gingivitis(G, n=30) and healthy periodontium (S, n=30). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine IL-38, IL-36γ, IL-17 and MMP-9 levels in the biological samples.

Conditions

• Mouth Diseases
• Periodontal Inflammation
• Periodontal Diseases

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	GCF obtaining	GCF obtaining: 2 GCF samples, 1 from the interproximal surface of 2 different teeth that bleeding on probing(-), were taken from each participant
DIAGNOSTIC_TEST	Saliva Obtaining	Saliva obtaining: 1,5 ml unstimulated whole saliva collected from each participant
DIAGNOSTIC_TEST	Serum obtaining	Serum obtaining : 8 ml of venous blood was collected from each participant and collecting blood centrifuged to obtain serum

Timeline

• Start date: 2020-06-01
• Primary completion: 2021-01-11
• Completion: 2021-01-11
• First posted: 2022-06-28
• Last updated: 2022-07-01

Locations

1 site across 1 country: Turkey (Türkiye)