NCT06758323

Acute Effects of Beetroot Juice and Caffeine Co-ingestion on Lower-limb Strength and Muscular Endurance in Females

Effect of Beetroot Juice Supplements with Caffeine Co-ingestion on Lower-limb Strength in Females

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 15 (actual)
• Sponsor: Jose Manuel Jurado Castro · Academic / Other
• Sex: Female
• Age: 18 Years – 35 Years
• Healthy volunteers: Accepted

Summary

The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are: * Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo? * How do the acute effects of beetroot juice interact with caffeine on physical performance? The study was conducted using a randomized, crossover design to evaluate the following four conditions: Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance. Participant Protocol Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.

Conditions

• Beetroot Juice
• Caffeine
• Placebo
• Beetroot Juice with Caffeine Co-ingestion

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Beetroot juice combined with caffeine (COM)	* 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session * 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session
DIETARY_SUPPLEMENT	Beetroot juice placebo with caffeine (CAF)	\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session
DIETARY_SUPPLEMENT	Beetroot juice with caffeine placebo (BRJ)	\- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session
OTHER	Beetroot placebo with caffeine placebo (PLA)	\- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session

Timeline

• Start date: 2024-08-01
• Primary completion: 2024-12-26
• Completion: 2024-12-26
• First posted: 2025-01-03
• Last updated: 2025-01-06

Locations

1 site across 1 country: Spain