Trials / Not Yet Recruiting

Not Yet RecruitingNCT06625645

Comparative Evaluation of Antimicrobial Efficacy, Clinical and Radiographic Success of Calcium Hydroxide, Chlorhexidine, and Modified Triple Antibiotic Paste in Different Combination Forms As Intracanal Medicaments Against Enterococcus Faecalis in Primary Teeth

Status: Not Yet Recruiting
Phase: N/A
Study type: Interventional
Enrollment: 32 (estimated)

Sponsor: Mahy Abdel Raouf Aly Kamar · Academic / Other
Sex: All
Age: 4 Years – 8 Years
Healthy volunteers: Not accepted

Summary

Comparing between different intracanal medications with different combination forms of Modified Triple Antibiotic Paste, Calcium Hydroxide and Chlorehexidine, regarding antimicrobial efficacy through bacterial reduction count of enterococcus Faecalis and regarding clinical and radiographic success in non vital primary teeth necessitating pulpectomy with follow up time 1 year.

Detailed description

1. Full medical and dental history will be obtained from the parents of the patients participating in this study. 2. A thorough clinical and radiographic examination of the tooth to be treated will be done. 3. The tooth will be anaesthetized using the inferior alveolar nerve block technique for lower molars and infiltration for upper molars 4. Before isolation, antisepsis of the oral cavity will be performed by rinsing for 1 min with 10 mL chlorhexidine gluconate mouth-wash 0.2 %. 5. The tooth will be properly isolated using rubber dam. 6. Access cavity will be performed using a round bur. 7. The root canals will be irrigated with 1 ml sterile saline solution. 8. Patency of the root canal will be obtained using stainless steel hand k- files size #15 9. Working length will be determined using apex locator 10. The first microbial samples (S1) will be collected to assess bacterial count of the root canals using 1 sterile paper point size 20, which will be inserted into the widest root canal for 1 min with pumping movements (McGurkin-Smith et al., 2005) 11. It will be placed inside sterile tubes containing a reduced transport medium of thioglycolate. 12. The canals will be thoroughly irrigated using 3ml syringe of 1.25% Sodium hypochlorite. A side-vented needle will be used to control the possibility of irrigant apical extrusion. 13. Mechanical preparation will be performed with rotary system for all the cases. 14. Irrigation with saline solution will be used to neutralize all the previously used solutions. 15. Then, a second microbial samples (S2) representing the antibacterial effect of the mechanical preparation will be obtained with the same procedure as the first sample (S1). 16. According to the randomization sequence, patients will be assigned to one of 4 groups: 1- A mix of modified triple Antibiotic Paste (M-TAP) and normal saline (experimental). 2- A mix of Ca(OH)2 and 2% CHX gluconate solution(experimental). 3- A mix of M-TAP and 2% CHX gluconate solution(experimental). 4- A mix of Ca(OH)2 and normal saline (control) 17) The canals will be dried and filled with intracanal medication plugged into the canal by using a plugger. 18\) At the end of the first visit a piece of sterile cotton will be placed in the pulp chamber. 19\) All teeth will be dressed with intermediate restorative material (IRM) as a temporary filling, in order to ensure proper sealing with no leakage of any oral fluids inside the root canal, which might disturb the action of the intracanal medication. 20\) After 7 days, the temporary restoration will be removed. 21) Disinfection of the operative field will take place as performed previously using the same protocol. 22\) Irrigation of the root canals with 2 mL of saline to flush out the medicaments and the third bacteriological sample (S3) will be taken out. 23\) Later, canals will be filled with Metapex (Metabiomed, Korea), followed by the placement of stainless-steel crowns (3M ESPE). 24\) The samples will be placed in sterile vials with thioglycolate (transport medium). The vials will be sealed tightly to avoid contamination and labeled. The labeled vials will be kept in a freezer and will be sent to the laboratory within 1 hour of collection, and samples will be assessed for total E. faecalis count (Qamar et al., 2023).

Conditions

Interventions

Type	Name	Description
COMBINATION_PRODUCT	Modified triple antibiotic paste (TAP) mixed with chlorehexidine	M-TAP with CHX 2%
COMBINATION_PRODUCT	Modified Triple Antibiotic Paste mixed with saline	M-TAP mixed with saline
COMBINATION_PRODUCT	Calcium Hydroxide mixed with chlorohexidine Intracanal medication	calcium hydroxide powder mixed with CHX 2% solution as intracanal medication
COMBINATION_PRODUCT	calcium hydroxide mixed with saline as intracanal medication	calcium hydroxide mixed with saline as intracanal medication for necrotic primary molars

Timeline

Start date: 2025-01-01
Primary completion: 2025-05-01
Completion: 2026-01-01
First posted: 2024-10-03
Last updated: 2024-10-17

Source: ClinicalTrials.gov record NCT06625645. Inclusion in this directory is not an endorsement.