NCT06602921

The Effects of Nitrate, Caffeine, and Cold Exposure on Cardiovascular Function.

Does Dietary Nitrate Supplementation Attenuate the Cardiovascular Strain Elicited by Caffeine and Cold Exposure in Healthy Individuals?

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 24 (actual)
• Sponsor: Loughborough University · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

Dietary inorganic nitrate supplementation may have positive implications on cardiovascular function. The aim of this project is to determine if nitrate supplementation can attenuate the effects that caffeine and cold exposure have on the cardiovascular system.

Detailed description

Acute caffeine consumption and cold exposure elicit negative effects on the cardiovascular system. Humans are regularly exposed to such conditions of increased cardiovascular strain, which may lead to elevated chronic cardiovascular risk. On the other hand, dietary inorganic nitrate supplementation is well established to improve cardiovascular function in healthy individuals. Thus, acute nitrate supplementation may compensate for the negative effects that caffeine and the cold place on the cardiovascular system. These effects will be measured using peripheral blood pressure, pulse wave analysis, heart rate variability, forearm blood flow, flow mediated dilation, and blood and saliva markers.

Conditions

• Nitrate
• Caffeine
• Cold Exposure

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	TruBeet inorganic beetroot juice	400 mg nitrate-rich beetroot powder, mixed with 300 mL water.
DIETARY_SUPPLEMENT	Caffeine	6 mg/kg caffeine powder in capsule form.
OTHER	Cold pressor test	3 min cold pressor test using the left foot in cold water (0-2 degree).

Timeline

• Start date: 2023-12-05
• Primary completion: 2024-11-01
• Completion: 2025-04-01
• First posted: 2024-09-19
• Last updated: 2025-09-12

Locations

1 site across 1 country: United Kingdom