NCT06064799

GCF miRNA-223 , -214 Levels in Smokers and Nonsmokers Periodontitis Patients

Expression of microRNA-223 and microRNA-214 in Gingival Crevicular Fluid of Smoker and Nonsmoker Periodontitis Patients, an Observational Diagnostic Accuracy Study

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 42 (actual)
• Sponsor: Fayoum University · Academic / Other
• Sex: All
• Age: 20 Years – 60 Years
• Healthy volunteers: Accepted

Summary

Objective: Periodontitis is a multifactorial disease that affects a wide range of populations. However, its pathogenesis remains unclear. miRNAs are now considered potential diagnostic markers for many inflammatory diseases. Thus, the aim of this study was to assess the expression of microRNA-223(miRNA-223) and microRNA-214 (miRNA-214) in gingival crevicular fluid (GCF) of smoker and nonsmoker with periodontitis. Materials and Methods: We conducted a prospective study among 42 participants: 14 healthy controls, 14 nonsmoker periodontitis participants, and 14 smoker with periodontitis. Eligibility criteria for inclusion were consecutive adults, aged 20-60 years, with stage III periodontitis grade B/C and no systemic diseases. All consenting participants had gingival crevicular fluid samples collected after diagnosis to assess miRNA-214 and -223 by quantitative real-time polymerase chain reaction assay.

Conditions

• Periodontitis, Adult

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	miRNA-223 & -214 detection	miRNA-223 \& -214 detection in GCF samples by PCR kits

Timeline

• Start date: 2023-01-01
• Primary completion: 2023-06-30
• Completion: 2023-06-30
• First posted: 2023-10-03
• Last updated: 2023-10-03

Locations

1 site across 1 country: Egypt