NCT06035029

Intestinal Flora and Metabonomics of Appendicitis

Research on Intestinal Flora and Metabonomics of Appendicitis in Children

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 30 (estimated)
• Sponsor: HongyiQu · Academic / Other
• Sex: All
• Age: 1 Year – 14 Years
• Healthy volunteers: Accepted

Summary

The goal of this observational study is to learn about the key bacterial flora and metabolites associated with appendicitis in children. The main questions it aims to answer are: * To screen out the key biomarkers of pediatric appendicitis. * What are the microbial differences in different parts of pediatric appendicitis patients. Participants will detect feces using 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology and differences of the fecal metabolites between healthy children and appendicitis children were analyzed using untargeted metabolomics based on Liquid chromatography-tandem mass spectrometry(LC-M S/MS) platform.Through the analysis of intestinal bacterial flora and metabolomics association and the differential analysis of intestinal bacterial flora in different parts of the case group, the key bacterial flora and metabolites were excavated.

Detailed description

Participants will detect feces using 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology and differences of the fecal metabolites between healthy children and appendicitis children were analyzed using untargeted metabolomics based on Liquid chromatography-tandem mass spectrometry(LC-M S/MS) platform. Differences in the intestinal flora of different parts of the case group: the intestinal flora of the case group in feces and rectum were detected by 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology, and the intestinal flora of different parts were analyzed and compared. Through the analysis of intestinal bacterial flora and metabolomics association and the differential analysis of intestinal bacterial flora in different parts of the case group, the key bacterial flora and metabolites were excavated.

Conditions

• Appendicitis
• Intestinal Metaplasia
• Metabonomics

Timeline

• Start date: 2023-04-03
• Primary completion: 2025-01-01
• Completion: 2025-07-01
• First posted: 2023-09-13
• Last updated: 2023-09-13

Locations

1 site across 1 country: China