NCT05997706

Unraveling the Klinefelter's Disease Physiopathology

Unraveling the Klinefelter's Disease Physiopathology by Organoid Model

• Status: Unknown
• Phase: N/A
• Study type: Interventional
• Enrollment: 20 (estimated)
• Sponsor: Cliniques universitaires Saint-Luc- Université Catholique de Louvain · Academic / Other
• Sex: Male
• Age: 5 Years – 50 Years
• Healthy volunteers: Not accepted

Summary

Organoid Model to unravel Klinefelter Syndrome infertility Klinefelter Syndrome (KS) is characterized by the presence of an extra chromosome X in male (47,XXY), it is the most frequent genetic cause of azoospermia in adult men. The investigators will isolate and expand spermatogonial cells from KS patients, then using an organoid model investigators will compare the behavior of these Spermatogonia from KS patients when interacting with four combinations of somatic cell types incorporated in the Extra Cellular Matrix hydrogel.

Detailed description

Men with Klinefelter Syndrome candidate for TESE or microTESE (testicular sperm extraction) for azoospermia. One extra testicular biopsy (1-2 mm) will be cryopreserved and used later for the cells isolation and culture.

Conditions

• Klinefelter Syndrome
• Infertility, Male

Interventions

Type	Name	Description
PROCEDURE	Testicular biopsy	Testicular biopsy is a clinical procedure for extraction of testicular tissue

Timeline

• Start date: 2018-06-12
• Primary completion: 2025-04-01
• Completion: 2025-04-01
• First posted: 2023-08-18
• Last updated: 2023-08-18

Locations

1 site across 1 country: Belgium