NCT05774925

Evaluation of Salivary Biomarker Levels of Individuals With Different Periodontal Diseases

Assessment of Saliva Leucine Rich α-2 Glycoprotein1 (LRG1) And C Reactive Protein (CRP) Levels In Individuals With Different Periodontal Diseases

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 60 (actual)
• Sponsor: Izmir Katip Celebi University · Academic / Other
• Sex: All
• Age: 20 Years – 60 Years
• Healthy volunteers: Accepted

Summary

The aim of this study is; detection of leucine rich α-2 glycoprotein1 (LRG1), c-reactive protein (CRP) levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine LRG and CRP levels in the biological samples.

Conditions

• Periodontal Diseases

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	saliva obtaining	The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.

Timeline

• Start date: 2021-12-08
• Primary completion: 2023-03-20
• Completion: 2023-04-10
• First posted: 2023-03-20
• Last updated: 2023-04-13

Locations

1 site across 1 country: Turkey (Türkiye)