NCT05772767

Modulation of Ciliogenesis in Glioma Stem Cells

Control of Growth and Invasiveness of Glioblastoma by Modulation of Ciliogenesis in Glioma Stem Cells. A Novel Target Against Glioblastoma for Precision Medicine. RF-2019-12368786

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 100 (actual)
• Sponsor: Fondazione Policlinico Universitario Agostino Gemelli IRCCS · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

The study aims at investigating the cilium-related transcriptome in patients-derived glioblastoma stem cells and the potential impact of modulation of cilium players in vitro, in vivo and ex vivo in glioblastoma brain organoids. Moreover, drugs inhibiting cilia disassembly will be tested. Finally, the potential prognostic role of a cilium-related gene expression signature in glioblastoma will be assessed.

Conditions

• Glioblastoma
• Glioma, Malignant

Interventions

Type	Name	Description
OTHER	Biological sample collection	Collection of tumor tissue and blood for stem cell culture and organoids generation.
OTHER	Dissecting ciliogenesis players	Assessment of cilium-related transcriptome in glioblastoma stem cells. Modulation of cilium-related genes and administration of cilium-targeted drugs to glioblastoma stem cells in vitro and ex vivo in glioblastoma brain organoids.
DIAGNOSTIC_TEST	Validating ciliogenesis players	Cilium-related signature will be studied in tumor tissue to evaluate its prognostic role.

Timeline

• Start date: 2021-02-15
• Primary completion: 2023-12-31
• Completion: 2024-07-13
• First posted: 2023-03-16
• Last updated: 2025-03-13

Locations

1 site across 1 country: Italy