NCT05073601

Thyroid Gland Dysfunction and Vitamin D Polymorphism in Keratoconus

Thyroid Gland Dysfunction and Vitamin D Receptor Polymorphism in Keratoconus

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 262 (actual)
• Sponsor: Mansoura University · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

This prospective study the thyroid gland dysfunction and vitamin D receptor polymorphism in keratoconus patients

Detailed description

This is a prospective observational study in which blood samples are collected from keratoconus patients looking for thyroid dysfunction and vitamin D levels in the serum and assessing the Vitamin D receptor polymorphism in comparison to healthy control.

Conditions

• Keratoconus

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	ELISA,DNA extraction	quantitative measurement of (Thyroid stimulating hormon (TSH), free triiodothyronine (FT3)\& Free tetraiodothyronine(FT4)) measured using ELISA . Serum 25-OH vitamin D measured using ELISA. Serum 25-OH vitamin D will be classified based on 2012 American Endocrine Society guidelines into deficient (\< 20ng/ml), insufficient ( 21 -30 ng/ml) and sufficient (\> 30 ng/ml).vitamin D receptor polymorphisms was done in 3 steps: a) DNA extraction from whole blood using Gene Jet gene DNA Purification kits . b) PCR: three Vitamin D receptor polymorphisms were tested \[Taq I (restriction enzymes according to single nucleotide polymorphism ) (rs731236), Apa I (rs7975232) and Bsm I (rs1544410)\] using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was amplified using certain primers

Timeline

• Start date: 2021-03-06
• Primary completion: 2021-07-01
• Completion: 2021-09-01
• First posted: 2021-10-11
• Last updated: 2021-10-21

Locations

1 site across 1 country: Egypt