NCT04660513

Obesity-Related Glycine Deficiency: Investigating a Long-standing Metabolic Paradox Using Bedside and Bench Approaches

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 42 (actual)
• Sponsor: Singapore General Hospital · Academic / Other
• Sex: All
• Age: 21 Years – 65 Years
• Healthy volunteers: —

Summary

Obesity, in addition to causing abnormal glucose and lipid metabolism, is also associated with altered plasma concentrations of multiple amino acids, including increased levels of branched-chain amino acids and decreased levels of glycine. The mechanisms and consequences of obesity- related glycine deficiency are unknown. The overall aim of this project is to comprehensively study glycine metabolic pathways in morbid obesity using stable-isotope tracer techniques in human subjects and validating kinetic findings using a cell model of oxidative stress. This will be a single-centre, observational study. 21 individuals with morbid obesity scheduled for bariatric surgery and 21 non-obese controls will be recruit. They will undergo different study visits and procedures and the human biological materials collected will be analysed for as per aims of the studies. We believe that the glycine metabolic pathways, possibly through the optimization of gluthathione (GSH) synthesis, may provide targets to develop novel therapeutic agents.

Detailed description

Metabolic tracers: 1,2-\[13C2\]-Glycine, 1,2-\[13C2\]-Glycine, 2,3,3,-\[2H3\]-Serine and \[2H5\]-Phenylalanine will be infused for quantification of various pathways associated with glycine metabolism.

Conditions

• Glycine Metabolism Disturbances
• Insulin Resistance
• Morbid Obesity
• Oxidative Stress

Interventions

Type	Name	Description
PROCEDURE	Bariatric surgery	Subjects with morbid obesity underwent bariatric surgery

Timeline

• Start date: 2018-12-08
• Primary completion: 2020-11-04
• Completion: 2020-11-04
• First posted: 2020-12-09
• Last updated: 2020-12-09

Locations

1 site across 1 country: Singapore