NCT04641026

Discovery of Biological Signatures for Cruciferous Vegetable Intake (Single Serving)

Discovery of Biological Signatures for Cruciferous Vegetable Intake: Integration of the Broccoli- and Host-derived Metabolome and the Microbiome

• Status: Active Not Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 83 (actual)
• Sponsor: Oregon State University · Academic / Other
• Sex: All
• Age: 18 Years – 60 Years
• Healthy volunteers: Accepted

Summary

The purpose of this project is to study the molecular and metabolic profiles in human plasma, urine and microbiome composition following the consumption of broccoli sprouts.

Detailed description

The investigators wish to examine metabolic and molecular markers found in blood and in urine after a single dose of sprouts, and evaluate the importance of the gut microbiome on these parameters. Some subjects will receive broccoli sprouts that are grown in deuterium oxide, a commonly used non-toxic, non-radioactive label for vegetables (Tang, Qin et al. 2005), so researchers can identify metabolites in blood and urine that are directly from consumed sprouts. The investigators plan to feed subjects a standardized breakfast and either broccoli sprouts or alfalfa sprouts (control) and look at the presence of metabolites and activity in the blood and urine 3, 6, 24, 48 and 72 hours following intake as well as changes in microbiome composition.

Conditions

• Diet, Healthy

Interventions

Type	Name	Description
OTHER	Broccoli sprouts	Subjects will consume one serving (about 1 cup) of broccoli sprouts with breakfast (bagel, cream cheese, and orange juice).
OTHER	Deuterium oxide-labelled broccoli sprouts	Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled broccoli sprouts with breakfast (bagel, cream cheese, and orange juice).
OTHER	Alfalfa sprouts	Subjects will consume one serving (about 1 cup) of alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice).
OTHER	Deuterium oxide-labelled alfalfa sprouts	Subjects will consume one serving (about 1 cup) of deuterium oxide-labelled alfalfa sprouts with breakfast (bagel, cream cheese, and orange juice).

Timeline

• Start date: 2021-02-22
• Primary completion: 2026-04-01
• Completion: 2026-12-01
• First posted: 2020-11-23
• Last updated: 2025-03-20

Locations

1 site across 1 country: United States