NCT04337385

Interrupting Sitting With High Intensity Exercise on Vascular Function in Adolescents Before and After a Mixed Meal

The Effect of Prolonged vs. Interrupted Sitting With High Intensity Interval Exercise on Lower Limb Vascular Function in Adolescents Before and After a Mixed Meal

• Status: Terminated
• Phase: N/A
• Study type: Interventional
• Enrollment: 6 (actual)
• Sponsor: University of Exeter · Academic / Other
• Sex: All
• Age: 12 Years – 15 Years
• Healthy volunteers: Accepted

Summary

This study aims to investigate the effect of interrupting a three hour bout of prolonged sitting with high intensity interval exercise on lower limb blood vessel function in adolescents before and after a mixed meal challenge.

Detailed description

Participants will be required to visit the laboratory on three separate occasions. The first of which will be a preliminary visit to collect anthropometric data (e.g. stature and body weight) and participants will complete a combined ramp-incremental and supra-maximal test to exhaustion on a cycle ergometer to determine the work rate for the exercise in the subsequent experimental visits. For the two experimental visits, participants will arrive to the laboratory after an overnight fast and an activPAL accelerometer will be attached to the midline of the participants' thigh for the duration of the visit to quantity sitting time. Lower limb macro and micro-vascular function will be assessed using a single protocol through flow mediated dilation of the popliteal artery and post-occlusive reactive hyperaemia in the capillaries of the calf. Participants will then either sit for three hours or have this three hour sitting period interrupted with high intensity interval cycling exercise at 30, 90 and 150 minutes. Vascular function will then be reassessed before participants will be given a mixed meal challenge (58.4 g fat and 53.4 g sugar) with a three hour postprandial period where the participants will remain seated for the duration. At the end of the postprandial period, vascular function will be reassessed. Blood pressure, heart rate and calf circumference will be assessed hourly throughout the experimental day. In addition, capillary blood samples will be collected at baseline, pre meal and each hour during the postprandial period and assessed for changes in plasma glucose, triglyceride, cholesterol and insulin concentrations. Participants will be transported to the toilet by wheelchair to limit the amount of walking undertaken during the sitting period and this activity will be quantified by the activPAL accelerometers.

Conditions

• Cardiovascular Risk Factor
• Endothelial Dysfunction

Interventions

Type	Name	Description
BEHAVIORAL	Interrupting 3 hours of prolonged sitting with high intensity interval exercise	Breaking up a prolonged bout of sitting in adolescents using high intensity interval exercise
BEHAVIORAL	3 hours of prolonged sitting	Participants will be asked to remain seated for 3 hours and refrain from moving their lower limbs

Timeline

• Start date: 2020-03-04
• Primary completion: 2020-03-13
• Completion: 2020-03-13
• First posted: 2020-04-07
• Last updated: 2021-05-25

Locations

1 site across 1 country: United Kingdom