NCT04126551

Mitochondrial Methylation in Type 2 Diabetes

Unraveling the Role of Mitochondrial DNA Methylation in Type 2 Diabetes

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 27 (actual)
• Sponsor: University of Arizona · Academic / Other
• Sex: All
• Age: 21 Years – 55 Years
• Healthy volunteers: Accepted

Summary

The overarching goal of this proposal is to determine whether DNA methylation of the mitochondrial DNA impairs mitochondrial function in insulin resistant states such as overweight/obesity and type 2 diabetes.

Detailed description

To determine whether differences in human skeletal muscle DNA methylation patterns in the mitochondrial and nuclear genome can explain the lower abundance of ETC and OXPHOS mRNA and protein observed in insulin resistant skeletal muscle of overweight/obese and type 2 diabetic participants. To determine whether patterns of human skeletal muscle DNA methylation in the mitochondrial and nuclear genome are predictive of ETC function. We will isolate skeletal muscle mitochondria from metabolically well-characterized lean insulin sensitive, overweight/obese insulin resistant nondiabetic and obese insulin resistant type 2 diabetic volunteers, and functionally evaluate each ETC complex (I - IV) and complex V (ATP synthase).

Conditions

• Overweight
• Obesity
• Diabetes Mellitus, Type 2
• Insulin Resistance

Interventions

Type	Name	Description
OTHER	Methylation status	Participants will be recruited, and muscle biopsies will be obtained for methylation analyses and measuring mitochondrial function

Timeline

• Start date: 2019-07-23
• Primary completion: 2023-12-31
• Completion: 2023-12-31
• First posted: 2019-10-15
• Last updated: 2024-05-07

Locations

1 site across 1 country: United States