Clinical Trials Directory

Trials / Completed

CompletedNCT03727646

Nicotinamide Riboside in LVAD Recipients

Pilot Study of Preoperative Nicotinamide Riboside (Vitamin B3) Supplementation in Patients Undergoing Elective Left Ventricular Assist Device (LVAD) Implantation

Status
Completed
Phase
EARLY_Phase 1
Study type
Interventional
Enrollment
5 (actual)
Sponsor
University of Washington · Academic / Other
Sex
All
Age
18 Years – 75 Years
Healthy volunteers
Not accepted

Summary

While preliminary data show that oral nicotinamide riboside (NR) supplementation increases myocardial levels of oxidized nicotine-adenine dinucleotide (NAD+) levels in mice, there has been no direct evidence that suggests oral NR increases NAD+ levels or improves mitochondrial function in human hearts. This Pilot Study is designed to obtain feasibility data for a planned, larger study testing the hypothesis that oral NR supplementation will increase myocardial NAD+ levels and improve cardiomyocyte mitochondrial function in participants with advanced heart failure planned for elective left ventricular assist device (LVAD) implantation. To demonstrate safety and feasibility of NR in this patient population, the investigators propose to enroll 5 participants planned for LVAD implantation in a Pilot Study of NR in which participants will receive NR, up-titrated over 3 days to a final NR dose of 1000mg twice daily. Blood and myocardial tissue analyses collected previously from age- and gender-matched LVAD recipients will serve as controls.

Detailed description

This Pilot Study will examine the following Aims: Aim 1: Enroll 5 participants scheduled for elective left ventricular assist device (LVAD) placement into an open-label study of nicotinamide riboside (NR). a. Participants will have labs (including safety panels) drawn at baseline (Day 1), then receive escalating doses of NR to a maximum dose of 1000mg twice daily by Day 3. Participants will be continued on NR at 1000mg twice daily until LVAD implantation surgery. On the morning of LVAD implantation Surgery (Day 5 or later), participants will have final labs drawn. Samples of fresh cardiac tissue removed from the left ventricular apex during LVAD implantation surgery will be collected in the operating room. The primary analyses will be performed on NR-treated participants who were on the maximum NR dose of 1000mg twice daily for at least 2 days prior to LVAD implantation surgery. The maximum duration of NR administration will be capped at 14 days. If the surgery doesn't happen by then, the participant will be withdrawn from the study. Aim 2: Determine the effect of NR (as compared to historical controls) on levels of the oxidized and reduced forms of nicotine-adenine dinucleotide (NAD+ and NADH, respectively), mitochondrial function and its regulation through modifications of the epigenome in the failing myocardium. 1. Measure NAD+ and NADH levels in the blood and myocardium of the participants. 2. Assess mitochondrial morphology and function in cardiac tissue using, respectively, electron microscopy (EM) and isolated mitochondria. 3. Determine protein acetylation in the mitochondrial and non-mitochondrial compartments and changes in nuclear gene regulation. Aim 3: Test the hypothesis that NR improves mitochondrial function and reduces inflammatory response in heart failure (HF) patients receiving NR (as compared to historical controls). 1. Measure mitochondrial function in peripheral blood mononucleated cells (PBMC). 2. Determine the inflammatory response in PBMC. 3. Compare effects on the circulating inflammasome vs. myocardial inflammation.

Conditions

Interventions

TypeNameDescription
DIETARY_SUPPLEMENTNicotinamide ribosidenicotinamide riboside supplied as 250mg capsules

Timeline

Start date
2018-09-26
Primary completion
2018-11-26
Completion
2018-11-26
First posted
2018-11-01
Last updated
2024-09-20
Results posted
2024-09-20

Locations

1 site across 1 country: United States

Regulatory

Source: ClinicalTrials.gov record NCT03727646. Inclusion in this directory is not an endorsement.