NCT03187860

Ciliation and Mucus Rheology Parameters Determined Via Air-liquid-interface Cell Cultures in Non-smoking, Smoking, COPD and Asthmatic Patients

Demonstration of the Diagnostic Character of the Rheology of Mucus

• Status: Terminated
• Phase: N/A
• Study type: Interventional
• Enrollment: 30 (actual)
• Sponsor: University Hospital, Montpellier · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

This is a prospective study comparing 4 groups: (1) non-smoking controls, (2) smokers without chronic obstructive pulmonary disease (COPD), (3) smokers with COPD, (4) severe asthma. Bronchial biopsy specimens from each subject will be obtained to produce air-liquid-interface cell cultures. These will then be used to make observations concerning cilia and mucus rheology. This is a first pilot study. The working hypothesis is that the largest group differences will be found for cilia densities; the latter metric was thus chosen as a primary criterion.

Conditions

• Pulmonary Disease, Chronic Obstructive
• Asthma
• Smoking

Interventions

Type	Name	Description
PROCEDURE	Bronchial Biopsy	Bronchial biopsies will be performed on all subjects.
OTHER	ALI culture	Air-liquid-interface cell cultures will be derived from bronchial biopsy specimens. We are expecting several replicate cell lines from each biopsy specimen.

Timeline

• Start date: 2019-06-19
• Primary completion: 2022-04-26
• Completion: 2022-12-26
• First posted: 2017-06-15
• Last updated: 2023-06-12

Locations

1 site across 1 country: France