NCT03178747

Tzanck Smear With Methylene Blue Stain for Herpes

A Comparative Study of Tzanck Smear With Methylene Blue Versus Giemsa Stain to Diagnose Herpes Simplex, Herpes Zoster and Varicella Zoster Skin Infections

• Status: Unknown
• Phase: Phase 3
• Study type: Interventional
• Enrollment: 150 (estimated)
• Sponsor: Chulalongkorn University · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

To compare the sensitivity of Tzanck smear with methylene blue stain versus traditional Giemsa stain in patients with herpes infection.

Detailed description

A smear from the lesional skin of the patients suspected having Herpes simplex, Herpes zoster and Varicella zoster skin infections will be collected (in a clinical routine setting). The specimen will be stained with methylene blue in addition to the Giemsa stain. Polymerase chain reaction study for herpes antigen will be performed in some of the specimens. The sensitivity, specificity, positive predictive value and negative predictive value will be calculated from the results of the study.

Conditions

• Herpes Simplex
• Herpes Zoster
• Varicella Zoster

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Methylene blue	working solution methylene blue
DIAGNOSTIC_TEST	Giemsa stain	working solution Giemsa stain (Wolbach's modification)

Timeline

• Start date: 2017-03-07
• Primary completion: 2017-12-07
• Completion: 2018-03-06
• First posted: 2017-06-07
• Last updated: 2017-08-04

Locations

1 site across 1 country: Thailand