Trials / Completed
CompletedNCT03129243
Sperm DNA Damage to Intracytoplasmic Sperm Injection Outcome
Relation of Sperm DNA Damage to Intracytoplasmic Sperm Injection Outcome in Couples With Male Infertility
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 120 (actual)
- Sponsor
- Assiut University · Academic / Other
- Sex
- Male
- Age
- 20 Years – 50 Years
- Healthy volunteers
- Not accepted
Summary
In the current era of assisted reproductive techniques where technology can help overcome defects in sperm function, the value of semen analysis has become even more inaccurate. Initial reports of intracytoplasmic sperm injection revealed its ability to bypass the natural selection process and enable men with severely impaired semen parameters to achieve both clinical pregnancy and live birth
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| DIAGNOSTIC_TEST | Sperm DNA integrity by flow cytometry | flowcytometric test where sperm DNA breaks are evaluated indirectly through the DNA denaturability. The assay measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ, followed by staining with the fluorescence dye acridine orange. By using a flow cytometer 5.000-10.000 sperm can be analyzed within few seconds and thus provide a less subjective result compared to the World health organization analysis where only 1-300 cells are analyzed. |
Timeline
- Start date
- 2018-04-01
- Primary completion
- 2019-09-15
- Completion
- 2019-10-30
- First posted
- 2017-04-26
- Last updated
- 2020-05-28
Locations
1 site across 1 country: Egypt
Source: ClinicalTrials.gov record NCT03129243. Inclusion in this directory is not an endorsement.