NCT02676362

Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Level

Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Levels: Sequential Sampling, Length of Sampling Time And Two Different Sampling Methods

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 20 (actual)
• Sponsor: Baskent University · Academic / Other
• Sex: All
• Age: 31 Years – 60 Years
• Healthy volunteers: Accepted

Summary

The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme activity by using intracrevicular and orifice methods.

Detailed description

Twenty adults (mean age of 41.8 years. ranged 31-60 years. 18 males. 2 females) with chronic periodontitis were enrolled and all completed the 3-day study. Three-hundred-sixty samples of GCF were harvested from the interproximal sites of each of the two maxillary non-molar teeth according to the orifice method. Each site was sampled three times with 1-min intervals between repeat samples. By using intracrevicular method, the other 360 samples of GCF were collected on the same sites following 10-minutes interval. In first day, 2nd day and 3rd day the length of sampling time in seconds and order were 5. 10. 30; 10. 30. 5 and 30. 5. 10. respectively. GCF elastase activity was determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide and was expressed as concentration (microU/microl) and total enzyme activity (microU).

Conditions

• Chronic Periodontitis

Interventions

Type	Name	Description
OTHER	Gingival crevicular fluid collection with filter paper	Gingival Crevicular Fluid Dynamics

Timeline

• Start date: 2008-03-01
• Primary completion: 2008-12-01
• Completion: 2008-12-01
• First posted: 2016-02-08
• Last updated: 2016-02-09