NCT02166567

Effect of Sorting of Apoptotic Spermatozoa on the Outcome of Assisted Reproduction

The Effect of Sorting of Non-apoptotic and Apoptotic Spermatozoa Using Flow Cytometry on the Outcome of Intracytoplasmic Sperm Injection.

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 100 (actual)
• Sponsor: University Hospital, Basel, Switzerland · Academic / Other
• Sex: All
• Age: 22 Years – 39 Years
• Healthy volunteers: Not accepted

Summary

In assisted reproductive technology (ART) semen needs to be processed. Commonly used methods rely on physical properties of the spermatozoa, such as the swim-up methods and density gradient centrifugation. It is now known that current processing of spermatozoa does not prevent the admixture of spermatozoa with damaged genetic material. The latter have a negative impact on the outcome of ART. The investigators developed a new method for the processing of spermatozoa with intact genetic material based on flow cytometry and sorting (FACS) and on staining using a deye, YoPro. The investigators aim at examining the effect of this novel method of sperm sorting on the outcome of ART.

Conditions

• Male Infertility

Interventions

Type	Name	Description
PROCEDURE	Staining of spermatozoa with the YoPro dye and sorting of the stained spermatozoa with FACS
PROCEDURE	Swim-up	Semen processing will be carried out with the conventional swim-up method.

Timeline

• Start date: 2013-05-01
• Primary completion: 2017-01-01
• Completion: 2017-11-01
• First posted: 2014-06-18
• Last updated: 2017-12-12

Locations

1 site across 1 country: Switzerland