Trials / Unknown
UnknownNCT01178463
Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia
- Status
- Unknown
- Phase
- —
- Study type
- Observational
- Enrollment
- 25 (estimated)
- Sponsor
- Hillel Yaffe Medical Center · Other Government
- Sex
- Male
- Age
- 18 Years
- Healthy volunteers
- Not accepted
Summary
The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| OTHER | Biospecimen staining | The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. |
| OTHER | Biospecimen staining | The sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. |
Timeline
- Start date
- 2010-01-01
- Primary completion
- 2010-05-01
- Completion
- 2010-10-01
- First posted
- 2010-08-10
- Last updated
- 2010-08-10
Locations
1 site across 1 country: Israel
Source: ClinicalTrials.gov record NCT01178463. Inclusion in this directory is not an endorsement.