NCT00898664

DNA Analysis of Tissue Samples From Patients With Head and Neck Cancer

Genetic Analysis of Head and Neck Squamous Cell Carcinoma

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 74 (actual)
• Sponsor: Vanderbilt-Ingram Cancer Center · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at DNA in tissue samples from patients with head and neck cancer.

Detailed description

OBJECTIVES: * Assess common genetic mutations in tumor and germline DNA samples from patients with head and neck squamous cell carcinoma (HNSCC). * Search for viral DNAs and relate this information to demographics, tumor characteristics, and patient prognosis. * Search for novel viral sequences and cancer-associated gene mutations in young nonsmoking HNSCC patients and in other distinct head and neck cancers. * Determine tumor suppressor gene mutations in HNSCC patients and relate this information to gene expression data. * Validate gene expression information using independent techniques such as real-time PCR on expressed sequences and immunohistochemistry. OUTLINE: Genetic mutation analysis and gene expression studies are performed on tissue samples from patients with head and neck squamous cell carcinoma. Techniques used include real time-PCR and immunohistochemistry.

Conditions

• Head and Neck Cancer

Interventions

Type	Name	Description
GENETIC	cytogenetic analysis
GENETIC	gene expression analysis
GENETIC	mutation analysis
GENETIC	polymerase chain reaction
OTHER	immunohistochemistry staining method
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2006-06-01
• Primary completion: 2008-04-01
• Completion: 2008-04-01
• First posted: 2009-05-12
• Last updated: 2013-04-02