NCT00873951

Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 26 (actual)
• Sponsor: Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed protein using a protein of good nutritional quality, i.e., casein. A second aim of this study was to assess the true ileal digestibility of dietary protein using different methods including the hydrolyzed casein method and to measure its possible effect on endogenous intestinal N losses in humans.

Conditions

• Healthy

Interventions

Type	Name	Description
OTHER	Intestinal and metabolic exploration	Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.
DIETARY_SUPPLEMENT	15N-labelled intact casein	standard mixed meal containing 15% of energy as 15N-labelled intact casein
DIETARY_SUPPLEMENT	15N-labelled hydrolyzed casein	standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein
DIETARY_SUPPLEMENT	mixture of AA	standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine

Timeline

• Start date: 2006-01-01
• Primary completion: 2006-11-01
• Completion: 2008-01-01
• First posted: 2009-04-02
• Last updated: 2025-10-06

Locations

1 site across 1 country: France