NCT00412906

Study of Gene Activity in Fat and Muscle in Diabetics and Healthy Controls

Study of Gene Expression in Fat and Muscle Tissue in Type 2 Diabetics and Healthy Controls During Experimental Endotoxemia

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 48 (estimated)
• Sponsor: Rigshospitalet, Denmark · Academic / Other
• Sex: Male
• Age: 25 Years – 80 Years
• Healthy volunteers: Accepted

Summary

The aim of the present protocol is to study gene activity in fat and muscle tissue in type 2 diabetics and healthy volunteers after injection of E. coli endotoxin.We hereby hope to gain insight in some mechanisms behind the association between inflammation and insulin resistance.

Detailed description

Numerous studies have shown an association between low-grade inflammation and insulin-resistance in type 2 diabetics. It is also well-known that septic patients often develop insulin-resistance. The pathogenetic mechanisms behind the association between inflammation and insulin-resistance is not fully understood. In this study we create an experimental inflammation by giving E. coli endotoxin 0,3 ng/kg iv to type 2 diabetics and healthy controls. Muscle biopsies are taken at 0, 2, 4 and 6 hours after injection, while fat biopsies are taken 0, 2, 4, 6 and 8 hours after endotoxin. mRNA for adiponectin, leptin, PPAR-gamma, PGC-1, PAI-1 and cytokines in tissue is later measured by RT-PCR. Blood samples are drawn on an hourly basis until 8 hours after endotoxin injection for common biochemical analyses including white blood cell count. Plasma is spared and later analyzed for cytokines, PAI-1 and VCAM-1/ICAM-1.

Conditions

• Healthy
• Type 2 Diabetes
• Endotoxemia

Interventions

Type	Name	Description
DRUG	Escherichia Coli Endotoxin

Timeline

• Start date: 2006-11-01
• Primary completion: 2008-04-01
• Completion: 2008-04-01
• First posted: 2006-12-19
• Last updated: 2008-05-21

Locations

1 site across 1 country: Denmark