NCT07533032

Dose-Response Effect of Phosphatidylserine on eSport Performance

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 80 (actual)
• Sponsor: Lindenwood University · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

This study will evaluate the effects of daily phosphatidylserine supplementation on electronic gaming performance, cognitive function, sleep quality, stress-related outcomes, and autonomic responses in healthy adult gamers. Participants will be enrolled in a randomized, double-blind, placebo-controlled, parallel-group study and assigned to receive placebo, 100 mg phosphatidylserine, or 200 mg phosphatidylserine daily for 6 weeks. Outcome measures will be assessed at baseline, Week 3, and Week 6 and will include electronic gaming performance, cognitive function, visual analog scale ratings, sleep quality questionnaires, hemodynamic responses, and heart rate variability. The study is designed to determine whether daily phosphatidylserine supplementation can improve performance and selected physiological and psychological outcomes during prolonged electronic gaming sessions.

Detailed description

Electronic gaming has grown substantially in popularity, with increasing interest in nutritional strategies that may improve gaming performance, cognitive function, sleep quality, and stress management. Phosphatidylserine is a phospholipid commonly derived from soy that is highly concentrated in neural tissue and has been investigated for its potential effects on cognition, stress-related responses, and mental performance. Despite growing interest in nutritional support for gamers, limited controlled research has evaluated the effects of supplementation on gaming-specific performance and related physiological outcomes. This study is a randomized, double-blind, placebo-controlled, parallel-group trial designed to examine the effects of phosphatidylserine supplementation on electronic gaming performance and associated cognitive, psychological, and physiological responses. Participants will be assigned to receive placebo, 100 mg phosphatidylserine, or 200 mg phosphatidylserine daily for 6 weeks. All participants and study personnel involved in testing and outcome assessment will remain blinded to treatment assignment throughout the study. Following screening and informed consent, eligible participants will complete a familiarization session during which they will practice the cognitive tests and become oriented to the gaming environment and procedures. Participants will then complete testing visits at baseline, Week 3, and Week 6. Prior to each testing visit, participants will be instructed to replicate their dietary intake for the preceding 24 hours, maintain their normal caffeine intake pattern, refrain from other stimulants not commonly consumed in their diet for 48 hours, avoid caffeine doses greater than 100 mg within 3 hours of testing, and complete a 3-hour fast before arrival. At each testing visit, body mass, resting heart rate, and resting blood pressure will be assessed. Participants will then complete sleep-related questionnaires, cognitive testing, and visual analog scales assessing fatigue, concentration, and focus. After ingestion of the assigned supplement dose, participants will undergo heart rate variability assessment and then complete two 60-minute electronic gaming sessions separated by interim testing. Hemodynamic responses, cognitive outcomes, and visual analog scale ratings will be reassessed after 60 minutes and again after 120 minutes of gaming. Heart rate variability will also be assessed following completion of the gaming session. Electronic gaming performance will be assessed using a standardized video game protocol with predefined performance metrics recorded after each gaming period. Cognitive outcomes will include the Stroop Color and Word Test, Trail Making Test Parts A and B, and Profile of Mood States. Sleep-related outcomes will be assessed using validated questionnaires, and autonomic function will be evaluated using heart rate variability metrics. Hemodynamic responses will include heart rate and blood pressure measured under standardized conditions. The primary objective of the study is to determine whether 6 weeks of phosphatidylserine supplementation improves electronic gaming performance, cognitive function, and subjective ratings related to fatigue, concentration, and focus compared with placebo. Secondary objectives include evaluating the effects of supplementation on sleep quality, heart rate variability, and hemodynamic responses.

Conditions

• Cognitive Function
• Gaming Performance

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Placebo	Participants will ingest a placebo supplement daily for 6 weeks. The supplement will be consumed with approximately 8 ounces of water at a consistent time each day.
DIETARY_SUPPLEMENT	Phosphatidylserine (100 mg)	Participants will ingest 100 mg of phosphatidylserine daily for 6 weeks. The supplement will be consumed with approximately 8 ounces of water at a consistent time each day.
DIETARY_SUPPLEMENT	Phosphatidylserine (200 mg)	Participants will ingest 200 mg of phosphatidylserine daily for 6 weeks. The supplement will be consumed with approximately 8 ounces of water at a consistent time each day.

Timeline

• Start date: 2021-06-15
• Primary completion: 2025-04-14
• Completion: 2025-04-14
• First posted: 2026-04-16
• Last updated: 2026-04-16

Locations

1 site across 1 country: United States