NCT07513727

Vitamin C for Gingival Depigmentation

Clinical and Histological Evaluation of Topical Vitamin C Application Using Three Modalities in Gingival Depigmentation

• Status: Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 30 (estimated)
• Sponsor: Al-Azhar University · Academic / Other
• Sex: All
• Age: 20 Years – 40 Years
• Healthy volunteers: Not accepted

Summary

The purpose of this study is to evaluate the clinical and histopathological efficacy of topical vitamin C applications using three modalities (topical gel, microneedling , and mesotherapy) in gingival depigmentation. Assessment includes clinical evaluation, CIELAB analysis via DOPI, and histopathological examination (Fontana-Masson staining).

Conditions

• Gingival Hyperpigmentation
• Gingival Melanin Pigmentation

Interventions

Type	Name	Description
DRUG	Topical Vitamin C ( control Arm)	Topical application of L-Ascorbic Acid gel (1000 mg in 5 ml distilled water with CMC and PEG), applied once daily on gingival hyperpigmented areas for 1 month.
PROCEDURE	Vitamin C mesotherapy	vitamin C mesotherapy was administered by delivering intra-mucosal microinjections of L-ascorbic acid into pigmented gingival tissues at the epithelium-connective tissue junction, providing targeted delivery to melanocyte-rich layers. . The treatment will be repeated 3 times with 1 week .
PROCEDURE	Microneedling technique	Gingival microneedling was performed using a Derma Pen A6 (12-needle row), with penetration depth adjusted to gingival thickness at 700 cycles/min (mode 6). Each gingival side was treated 30-40 seconds per tooth until bleeding points appeared.

Timeline

• Start date: 2025-07-29
• Primary completion: 2026-05-01
• Completion: 2026-05-01
• First posted: 2026-04-07
• Last updated: 2026-04-07

Locations

1 site across 1 country: Egypt