NCT07503860

Indirect Biomarkers and Exercise Induced Muscle Damage

Repeated Bout Effect and Indirect Biomarkers After Eccentric Exercise Induced Muscle Damage

• Status: Active Not Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 48 (actual)
• Sponsor: Vassilis Paschalis · Academic / Other
• Sex: Male
• Age: 18 Years – 30 Years
• Healthy volunteers: Accepted

Summary

The purpose of this study is to investigate the magnitude of changes in biomarkers of exercise-induced muscle damage following an acute bout of eccentric exercise of the knee extensors performed on an isokinetic dynamometer. Moreover, these biomarkers will be assessed at different time points after the initial intervention, checking the repeated bout effect phenomenon.

Detailed description

Volunteers will visit the laboratory a total of two times. During the first visit, in all participants will be assessed indirect markers of exercise-induced muscle damage (i.e., peak torque, delayed-onset muscle soreness, range of motion, rate of force development), blood parameters (i.e., creatine kinase and c-Reactive Protein), and muscle oxygenation using near infrared spectroscopy. Afterwards, participants will perform an eccentric exercise protocol for the knee extensors consisting of 5 sets of 15 maximal-intensity repetitions at an angular velocity of 60°/s. The second visit will be at different time points between participant, where the indirect mauls damage biomarkers will be reassessed before and after the same isokinetic eccentric exercise (i.e., 5 sets of 15 maximal-intensity repetitions at an angular velocity of 60°/s).

Conditions

• Exercise Induced Muscle Damage

Interventions

Type	Name	Description
OTHER	Eccentric Exercise	Eccentric exercise will be performed using an isokinetic dynamometer. It will be consisted of 5 set of 15 eccentric contraction of the knee extensors at an angular velocity of 60o/s. The intensity will be maximal voluntary.

Timeline

• Start date: 2025-08-22
• Primary completion: 2025-11-27
• Completion: 2026-07-15
• First posted: 2026-03-31
• Last updated: 2026-03-31

Locations

1 site across 1 country: Greece