NCT07439614

Comparison of Root Canal Desinfection Protocols

Comparison of Different Root Canal Disinfection Protocols for Bacterial Reduction in Teeth With Apical Periodontitis Using Two Analytical Microbiological Methods: a Randomized Clinical Study

• Status: Not Yet Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 50 (estimated)
• Sponsor: State University of New York at Buffalo · Academic / Other
• Sex: All
• Age: 18 Years – 70 Years
• Healthy volunteers: Accepted

Summary

Evaluate and compare the disinfecting efficacy of two supplementary antimicrobial protocols in teeth with apical periodontitis

Detailed description

This study aims to evaluate and compare the disinfecting efficacy of two supplementary antimicrobial protocols in teeth with apical periodontitis: Er,Cr:YSGG laser activated irrigation and ultrasonic activation using a piezoelectric ultrasonic device (Dentsply International Inc., York, PA, USA) and Irrisafe tip (Satelec/Acteon, Merignac, France), comparing the detection of bacteria before and after instrumentation. Secondary aims are to assess presence of bacteria before and after chemomechanical preparation using two microbiological methods: * ATP level analysis using the Endocator device * Quantitative PCR (qPCR) for total bacterial quantification.

Conditions

• Periapical Periodontitis

Interventions

Type	Name	Description
DEVICE	Desinfection device	Desinfection protocol with (LAI): Er,Cr:YSGG laser (2780 nm) delivered with a radial-firing tip.
DEVICE	Analytical microbiological methods	Endocator: Measures ATP levels (Endoscore and RLU) to rapidly assess bacterial load in less than one minute.

Timeline

• Start date: 2026-02-01
• Primary completion: 2026-06-01
• Completion: 2026-12-01
• First posted: 2026-02-27
• Last updated: 2026-02-27