NCT07369518

Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae

Effect of Ceftazidime-Avibactam Against Carbapenemase-Positive Klebsiella Pneumoniae Causing Health Care-Associated Infections

• Status: Not Yet Recruiting
• Phase: —
• Study type: Observational
• Enrollment: 100 (estimated)
• Sponsor: Sohag University · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Accepted

Summary

Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections * analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.

Detailed description

Carbapenem-resistant Enterobacterales (CRE) poses an urgent global public health threat, with more than 1,100 documented deaths, as reported in a 2019 antibiotic resistance publication by the U.S. Centers for Disease Control and Prevention. Carbapenem-resistant Enterobacterales, including carbapenem-resistant Klebsiella pneumoniae (CRKP), is associated with higher mortality compared with infections caused by carbapenem-susceptible Enterobacterales infections Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections * analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.

Conditions

• ISOLATION OF KLEB. by Culture on macConkey Agar
• Antibiotic Sensitivity Testing of Different Antibiotics According to Clsi 25
• Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae
• Detection of Resistance Gene in Isolates Using Conventional Pcr
• Detection of Some Virulence Genes Using Conventional Pcr

Interventions

Type	Name	Description
GENETIC	. - Genotypic detection of carbapenemase genes by conventional PCR.	Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections - analysisThe resistance pattern of the isolates will be detected by disc diffusion method. - phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.

Timeline

• Start date: 2026-01-01
• Primary completion: 2026-12-01
• Completion: 2026-12-01
• First posted: 2026-01-27
• Last updated: 2026-01-27

Locations

1 site across 1 country: Egypt