NCT07367451

Effects of Propolis Flavonoids on TLR4 Signaling and Inflammation in Human PDLSCs In Vitro

Effect of Propolis-Derived Flavonoid Extract on TLR4 Signaling and Inflammatory Cytokine Expression in Human Periodontal Ligament Stem Cells (PDLSCs): An In-Vitro Study

• Status: Not Yet Recruiting
• Phase: —
• Study type: Observational
• Enrollment: 20 (estimated)
• Sponsor: Cairo University · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

This in-vitro study evaluated the effects of a flavonoid-rich propolis extract on Toll-like receptor 4 (TLR4) signaling and inflammatory cytokine expression in human periodontal ligament stem cells (PDLSCs). PDLSCs were treated with propolis flavonoids under baseline conditions and following lipopolysaccharide (LPS)-induced inflammation, with untreated cells serving as controls. TLR4 gene expression was analyzed using PCR, while levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were quantified by ELISA. Cell viability and cytotoxicity at different extract concentrations were assessed using the MTT assay. The study aimed to determine the immunomodulatory and cytocompatible potential of propolis flavonoids in regulating inflammatory responses in PDLSCs.

Conditions

• Inflammatory Cytokine Expression

Interventions

Type	Name	Description
DRUG	Flavonoid extract from propolis	Preparation of Propolis Flavonoid Extract Concentrations for testing: 5 µg/mL 10 µg/mL 20 µg/mL
OTHER	no medication added	No medication will be added to the second group

Timeline

• Start date: 2026-01-25
• Primary completion: 2026-01-30
• Completion: 2026-02-10
• First posted: 2026-01-26
• Last updated: 2026-02-05