NCT07334054

Effect of Vitamin D and Branched-chain Amino Acids on Physical Performance and Biomarkers of Muscle Fatigue in Runners

Effect of Vitamin D and Branched-chain Amino Acids on Physical Performance and Biomarkers of Muscle Fatigue in Runners: an 8-week Randomized, Double-blind

• Status: Not Yet Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 64 (estimated)
• Sponsor: Pedro Julian Flores Moreno · Academic / Other
• Sex: Male
• Age: 25 Years – 44 Years
• Healthy volunteers: Accepted

Summary

Muscle fatigue caused by physical training is understood as a condition related to the inability to maintain action potential, derived from the alteration in skeletal muscle homeostasis. In long-distance recreational runners, prolonged physical work is performed while maintaining the level of intensity, where a level of fatigue intervenes, which overlaps and generates tiredness to execute the movement continuously. In the last 10 years, sports supplementation has been explored as an aid to increase physical performance, improve muscle recovery and prevent sports injuries.

Detailed description

Objective: To analyze the effects of an 8-week training program (TP) with vitamin D and branched-chain amino acids (BCAA) supplementation on physical performance and biomarkers of muscle fatigue in long-distance recreational runners. Materials and methods: An 8-week randomized controlled clinical trial. Participants will be 64 men aged 25 to 44 years, distributed into 4 intervention groups. Group A: Training + BCAA + Vitamin D. Group B: Training + BCAA + Placebo. Group C: Training + Vitamin D + Placebo. Group D: Training + Placebo + Placebo. Biomarkers of muscle fatigue Interleukin 6 (L-6), Creatine Kinase (CK), Lactate (Lact), Testosterone and Cortisol ratio and serum vitamin D (25(OH)D) and physical performance will be assessed by estimating Maximal oxygen consumption (VO2max) and (Maximal aerobic speed) MAS.

Conditions

• Healthy Adult Male
• Runners

Interventions

Type	Name	Description
OTHER	Training program	Aerobic training program (60-90% of maximum aerobic speed)
DIETARY_SUPPLEMENT	Branched-Chain Amino Acids	Branched-chain amino acids (5 gr/day)
DIETARY_SUPPLEMENT	Vitamin D	Vitamin D (1000 IU/day)
OTHER	Placebo 1	Placebo 1 (5gr/day)
OTHER	Placebo 2	Placebo 2 (1 tablet/day)

Timeline

• Start date: 2026-01-01
• Primary completion: 2026-03-30
• Completion: 2026-06-01
• First posted: 2026-01-12
• Last updated: 2026-01-12

Locations

1 site across 1 country: Mexico