NCT07303647

Genes Associated With Bone Metabolism in the Saliva During Orthodontic Treatment

Expression of Genes Related to Bone Metabolism in Saliva of Patients During Early Fixed Orthodontic Treatment: a Prospective Clinical Study

• Status: Active Not Recruiting
• Phase: —
• Study type: Observational
• Enrollment: 24 (actual)
• Sponsor: Kurdistan Higher Council of Medical Specialties · Academic / Other
• Sex: All
• Age: 15 Years – 25 Years
• Healthy volunteers: Not accepted

Summary

Understanding the biological events during fixed orthodontic treatment is essential for optimizing treatment strategies, predicting patient response, and minimizing adverse effects. Most studies on bone remodeling have used invasive sampling methods such as tissue biopsies or serum collection; these methods cannot be used for routine clinical monitoring. Saliva is a simple medium that can reflect changes in local periodontal and bone conditions, it is also non-invasive and cheap. There is little evidence about the temporal expression of genes related to bone metabolism (RANKL, OPG, ALP, TRAP, RUNX2) in saliva during orthodontic therapy. This study will help advance the understanding of biological responses during orthodontic tooth movement and explore whether saliva can be an appropriate diagnostic medium for monitoring bone remodeling in orthodontic patients

Conditions

• Gene Expression Profiling
• Orthodentic Appliances

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	PCR	Unstimulated whole saliva collection SOP (time of day, fasting, avoid toothbrushing immediately prior). RNA stabilization and extraction (saliva RNA kits). cDNA synthesis and quantitative RT-PCR (or RNA-seq if budget allows). Housekeeping genes for normalization (e.g., GAPDH, ACTB - validate stability in saliva). Analysis method: ΔΔCt → fold change.

Timeline

• Start date: 2025-10-12
• Primary completion: 2026-02-01
• Completion: 2026-02-01
• First posted: 2025-12-26
• Last updated: 2025-12-26

Locations

1 site across 1 country: Iraq