NCT07160894

Evaluation of Nucleotide-Binding Oligomerization Domain-Containing Protein (NOD) 1 and NOD 2

Evaluation of Nucleotide-Binding Oligomerization Domain-Containing Protein (NOD) 1 and NOD 2 in Different Periodontal Conditions

• Status: Not Yet Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 75 (estimated)
• Sponsor: Medipol University · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

The host immune response to microorganisms is primarily recognized by pattern recognition receptors (PRRs), including toll-like receptors and nucleotide-binding oligomeric domain (NOD) receptors (NLRs). The aim of this study was to examine the relationship between periodontal disease and NOD in serum and saliva samples.

Detailed description

Healthy participants (n:25), gingivitis (n:25) patients and stage III, grade B periodontitis (n:25) patients enrolled for this study. Clinical periodontal parameters and salivary flow rate were noted. Serum and saliva samples were collected to measure NOD1, NOD2, IL-10 and IL-6 levels.

Conditions

• Periodontal Diseases Inflammation

Interventions

Type	Name	Description
OTHER	Saliva and Serum sampling	Saliva were collected to analyze the selected markers as unstimulated samples during the early hours of the day. The saliva was centrifuged and then transferred into Eppendorf tubes. Venous puncture was performed after saliva collection and 5 mL of blood samples were collected by qualified staff from each participant.Saliva and serum were then stored at -80 °C until analysis.
OTHER	Saliva and Serum sampling	ELISA method will be used to measure IL-10 and IL-6 values in collected saliva and serum samples. For ELISA analysis, a commercial kit (Sunred Bio, Shanghai, China) pre-coated with human selected antibodies will be used.

Timeline

• Start date: 2025-09-15
• Primary completion: 2026-03-12
• Completion: 2026-07-20
• First posted: 2025-09-08
• Last updated: 2025-09-08

Locations

1 site across 1 country: Turkey (Türkiye)