NCT07112677

Seroprevalence of Brucella Antibodies in High-Risk Patients With Low Back Pain: A Case-Control Study

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 100 (actual)
• Sponsor: Elham Ahmed Hassan · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

The goal of this observational case-control study is to assess the presence of Brucella antibodies in patients with low back pain who are at high risk for brucellosis exposure, such as those with animal contact or consumption of unpasteurized dairy products. The main question it aims to answer is: Is there a significant association between Brucella seropositivity and low back pain in high-risk individuals? Researchers will compare patients with low back pain to age- and sex-matched healthy controls without back pain but with similar exposure risks, to see if Brucella antibodies are more frequent in the patient group. Participants will: Undergo a detailed clinical history and physical examination Provide blood samples for serological testing using ELISA for Brucella IgG/IgM/IgA Be evaluated for other symptoms such as fever, fatigue, and joint pain

Conditions

• Brucellosis
• Low Back Pain

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Brucella Antibody Testing (ELISA)	Serological testing for Brucella antibodies using the SERION ELISA classic Brucella IgG/IgM/IgA kit (Institut Virion\\Serion GmbH, Würzburg, Germany). The test detects Brucella-specific IgG, IgM, and IgA antibodies in serum samples to assess current or past exposure. All samples were processed and interpreted according to the manufacturer's instructions. This diagnostic intervention was used for both the low back pain group and the control group.

Timeline

• Start date: 2024-02-01
• Primary completion: 2025-02-28
• Completion: 2025-02-28
• First posted: 2025-08-08
• Last updated: 2025-08-08

Locations

2 sites across 1 country: Egypt