NCT07103889

Effect of Acute Exercise on Biochemical Parameters in Normal-Weight and Overweight/Obese Adolescent.

Assessment of Biochemical, Metabolic, Inflammatory, and Hematological Parameters Following Acute Exercise in Normal-Weight and Overweight/Obese Adolescent Girls.

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 35 (actual)
• Sponsor: High Institute of Sports and Physical Education of Kef · Academic / Other
• Sex: Female
• Age: 13 Years – 18 Years
• Healthy volunteers: Not accepted

Summary

The goal of this interventional clinical trial is to compare the acute physiological and biochemical responses to maximal physical exercise (Spartacus test). in two groups of adolescent girls: in normal-weight and overweight/obese adolescent. The main question it aims to answer: * Does Spartacus test significantly alter glucose metabolism and lipid profile in normal-weight and overweight/obese adolescent? * How do inflammatory markers such as C-Reactive Protein \[CRP\], Erythrocyte Sedimentation Rate \[ESR\], respond immediately after and 30 minutes following maximal effort? * Are there significant changes in liver enzymes (Aspartate Aminotransferase \[AST\], Alanine Aminotransferase \[ALT\]), muscle damage indicators (creatine kinase \[CK\], Lactate Dehydrogenase \[LDH\]), and electrolyte balance post-exercise? Participants will undergo a baseline blood draw before performing the Spartacus test , a progressive maximal exercise test. Blood samples will then be collected immediately after the test and again 30 minutes post-exercise to evaluate changes in: Glucose metabolism (fasting glucose), Lipid profile (total cholesterol, High-Density Lipoprotein \[HDL\], Low-Density Lipoprotein \[LDL\], triglycerides), Liver and muscle enzymes (Aspartate Aminotransferase \[AST\], Alanine Aminotransferase \[ALT\], Creatine kinase \[CK\], Lactate Dehydrogenase \[LDH\]), Renal function markers (creatinine, urea), Inflammatory markers (C-Reactive Protein \[CRP\], Erythrocyte Sedimentation Rate \[ESR\]), Hematological profile (complete blood count: White Blood Cells \[WBC\], Red Blood Cells \[RBC\], Hemoglobin \[Hb\], Hematocrit \[Hct\], platelets), Electrolytes (sodium, potassium, chloride), Uric acid, calcium, phosphorus, magnesium, iron.

Detailed description

This is an interventional clinical trial designed to evaluate the acute physiological and biochemical responses to maximal physical exercise (Spartacus test) in normal-weight and overweight/obese adolescent. The study aims to investigate how a single session Spartacus test affects key metabolic, inflammatory, hepatic, muscular, renal, and hematological parameters. Participants will be divided into two groups: Group 1: Normal-weight adolescent girls Adolescent girls with a body mass index (BMI) between the 5th and \<85th percentile for age. Group 2: Overweight/obese adolescent girls Adolescent girls with a BMI ≥ 85th percentile for age. The protocol involves running on a simulated rectangular course (750 m²), with increasing speed every 3 minutes (starting at 7 km/h and increasing by 1 km/h each stage). Participants must reach a marked cone corresponding to the new speed within 15 seconds, followed by a 15-second rest period before the next stage begins. The test continues until voluntary exhaustion or inability to maintain the required pace. Blood samples will be collected at three time points: Baseline (Time 0): Before the start of the exercise. Immediately post-exercise (Time 1): Within 5 minutes after completion of the test. 30 minutes post-exercise (Time 2): To assess early recovery kinetics. The following biomarkers will be measured at each time point: Metabolic profile: Fasting glucose, lipid panel (total cholesterol, HDL, LDL, triglycerides), uric acid Liver function: AST, ALT, LDH, CK Renal function: Creatinine, urea Inflammatory markers: C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) Hematological profile: Complete blood count (CBC), including white blood cell differential, hemoglobin, hematocrit, red blood cell indices, and platelet count Electrolytes: Sodium (Na⁺), potassium (K⁺), chloride (Cl-) Minerals and trace elements: Calcium, phosphorus, magnesium, iron

Conditions

• Obese Adolescents
• Overweight

Interventions

Type	Name	Description
BEHAVIORAL	Spartacus Test	The Spartacus test was conducted on a 750 m (75 × 10 m) rectangular course marked with cones at regular intervals to indicate different speeds (from 7 to 18 km/h). Each participant began running at 7 km/h, with speed increasing by 1 km/h every three minutes.During the 3-minute intervals (for each speed stage), participants had 15 seconds to reach the corresponding marker, followed by 15 seconds of rest. Participants had to adjust their running pace to reach the target cone in synchrony with an audio signal. The test ended when a participant failed to reach the next cone within the given time. This test is validated for assessing maximal aerobic capacity (MAS) in obese adolescents. During the Spartacus test, maximal heart rate (HRmax) was recorded using a heart rate monitor (S810; Polar, Kempele, Finland).

Timeline

• Start date: 2025-08-10
• Primary completion: 2025-08-13
• Completion: 2025-08-13
• First posted: 2025-08-05
• Last updated: 2025-09-11

Locations

1 site across 1 country: Tunisia