NCT07008352

Amino Acid Metabolism Before and After Bariatric Surgery

Examination of Preoperative and Postoperative Serum Amino Acid Metabolism in Individuals Undergoing Bariatric Surgery

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 100 (actual)
• Sponsor: Harran University · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

This observational study aims to investigate the changes in serum amino acid metabolism before and after bariatric surgery in obese individuals. Blood samples will be collected from 50 patients at baseline (preoperative), and at 1 and 3 months postoperatively. An additional 50 healthy individuals will serve as the control group. Amino acid profiling will be conducted using LC-MS/MS. The study seeks to determine how bariatric surgery affects metabolic pathways associated with amino acid regulation.

Detailed description

Obesity is a major global health problem that significantly increases the risk of metabolic disorders. Bariatric surgery is one of the most effective interventions for sustained weight loss in morbidly obese patients. However, the metabolic effects of such surgery on amino acid profiles are not fully understood. In this prospective observational study, a total of 100 participants will be enrolled: 50 patients undergoing bariatric surgery and 50 healthy controls. Serum samples will be collected from the patient group preoperatively, and at 1 and 3 months after surgery. The control group will provide one-time blood samples. All serum samples will be centrifuged and stored at -80°C until analysis. Free amino acid profiles will be determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The primary outcome is to assess the change in amino acid concentrations over time in surgical patients, and to compare them with healthy controls. Secondary analyses will explore correlations between amino acid levels and body mass index (BMI). Data analysis will be performed using SPSS and MetaboAnalyst 5.0. This study will help clarify how bariatric surgery influences amino acid metabolism and may offer insights into the metabolic mechanisms underlying postoperative recovery and weight regulation.

Conditions

• Obesity, Morbid

Interventions

Type	Name	Description
PROCEDURE	Blood sample collection for amino acid profiling	Blood samples (5 mL) will be collected from participants in both groups. In the bariatric surgery group, samples will be collected at three time points: preoperatively, and at 1 and 3 months postoperatively. For the control group, a single sample will be obtained. Serum will be separated, stored at -80°C, and analyzed for free amino acid profiles using LC-MS/MS.

Timeline

• Start date: 2025-06-11
• Primary completion: 2025-10-02
• Completion: 2025-11-03
• First posted: 2025-06-06
• Last updated: 2025-11-26

Locations

1 site across 1 country: Turkey (Türkiye)