NCT06986044

EVALUATION OF SALIVA AND SERUM HEME OXYGENASE, ARYLESTERASE AND NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR 2 LEVELS IN PATIENTS WITH STAGE III PERIODONTITIS

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 74 (actual)
• Sponsor: Saglik Bilimleri Universitesi · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

Periodontal disease is a chronic and progressive inflammatory disease in which the hard and soft tissues that support the teeth are damaged. It is caused by the interaction between harmful bacteria and the body's immune responses, and most periodontal tissues are damaged by the body's abnormal response to these microorganisms and their products. When bacteria enter the body, immune cells (neutrophils) produce reactive oxygen species (ROS) in a process called the "respiratory burst". These ROS damage cells, causing tissue destruction through a variety of mechanisms, including DNA damage, fat oxidation and protein damage. Studies have shown that neutrophils from individuals with periodontal disease produce more ROS than neutrophils from healthy individuals. High amounts of ROS lead to oxidative damage to gum tissue, periodontal ligament and alveolar bone. Oxidative stress occurs when antioxidants in the body are insufficient or when high levels of ROS are present. Therefore, disruption of the balance between oxidant and antioxidant activities is considered an important cause of oxidative damage in periodontal tissues. Parameters such as total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) are used to determine oxidative stress. Furthermore, some enzymes such as arylesterase (ARE), heme oxygenase (HO) and nuclear factor erythroid 2-related factor 2 (NRF-2) are involved in defense mechanisms against oxidative stress. Many recent studies have shown a strong association between oxidative stress and periodontal disease.

Detailed description

This study was divided into 2 groups, 37 systemically and periodontally health individuals and 37 systemically healthy individuals with stage III grade B periodontitis were included. Clinical periodontal parameters plaque index (PI), gingival index (GI), probing pocket depth (SCD), bleeding on probing (BOP) and clinical attachment level (CAL) were recorded and saliva and serum samples were collected. ELISA method was used for analyses of TOS, TAS, OSI, ARE, HO-1, NRF-2 levels.

Conditions

• Periodontal Health
• Periodontitis Stage III

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Serum Total Oxidant Status	Serum total oxidant status
DIAGNOSTIC_TEST	Salivary Total Oxidant Status	Salivary Total Oxidant Status
DIAGNOSTIC_TEST	Serum Total Antioxidant Status	Serum Total Antioxidant Status
DIAGNOSTIC_TEST	Salivary Total Antioxidant Status	Salivary Total Antioxidant Status
DIAGNOSTIC_TEST	Serum Oxidative Stress Index	Serum Oxidative Stress Index
DIAGNOSTIC_TEST	Salivary Oxidative Stress Index	Salivary Oxidative Stress Index
DIAGNOSTIC_TEST	Serum Arylesterase	Serum Arylesterase
DIAGNOSTIC_TEST	Salivary Arylesterase	Salivary Arylesterase
DIAGNOSTIC_TEST	Serum Heme Oxygenase	Serum Heme Oxygenase
DIAGNOSTIC_TEST	Salivary Heme Oxygenase	Salivary Heme Oxygenase
DIAGNOSTIC_TEST	Serum Nuclear Factor Erythroid 2 Related Factor 2	Serum Nuclear Factor Erythroid 2 Related Factor 2
DIAGNOSTIC_TEST	Salivary Nuclear Factor Erythroid 2 Related Factor 2	Salivary Nuclear Factor Erythroid 2 Related Factor 2
DIAGNOSTIC_TEST	Gingival Index	Gingival Index
DIAGNOSTIC_TEST	Plaque Index	Plaque Index
DIAGNOSTIC_TEST	Bleeding on Probing Index	Bleeding on Probing Index
DIAGNOSTIC_TEST	Clinical Attachment Loss	Clinical Attachment Loss
DIAGNOSTIC_TEST	Pocket Deep	Pocket Deep

Timeline

• Start date: 2023-05-01
• Primary completion: 2024-05-01
• Completion: 2024-09-15
• First posted: 2025-05-22
• Last updated: 2025-05-22

Locations

1 site across 1 country: Turkey (Türkiye)