NCT06972134

Synergistic Effects of Natural Oils and Herbal Extracts on Cholesterol Gallstone Dissolution: In Vitro Comparison With Rowachol®

Synergistic Effects of Sesame Oil, Extra Virgin Olive Oil, Psyllium Extract, and Dandelion Extract on Cholesterol Gallstone Dissolution: An In Vitro Comparative Study Against Rowachol®

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 55 (actual)
• Sponsor: University of Duhok · Academic / Other
• Sex: All
• Age: 40 Years – 50 Years
• Healthy volunteers: Not accepted

Summary

Objective: This study aims to evaluate the effectiveness of a natural mixture (sesame oil, extra virgin olive oil, psyllium extract, and dandelion extract) in dissolving cholesterol gallstones compared to the standard medication, Rowachol®. Methods: In laboratory experiments, 70 cholesterol gallstones obtained from 55 patients were divided into seven groups. Each group was treated with different combinations of the natural ingredients or Rowachol®. The stones were monitored for 144 hours to measure dissolution rate and cholesterol release.

Conditions

• Cholesterol Gallstone Dissolution

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Sesame Oil (SO) at 100 mg/mL in dissolving fluid.	Pure Sesamum indicum oil (cold-pressed, unrefined) dissolved in BioIVT bile juice at 100 mg/mL. Prepared by homogenization (30 min, 37°C). Used as monotherapy.
DIETARY_SUPPLEMENT	Extra Virgin Olive Oil (EVOO) at 100 mg/mL in dissolving fluid.	Pure Olea europaea oil (first cold-pressed, acidity ≤0.8%) dissolved in BioIVT bile juice at 100 mg/mL. Prepared by vortex mixing (15 min, 25°C). Used as monotherapy.
DIETARY_SUPPLEMENT	50% Sesame Oil (SO) + 50% Extra Virgin Olive Oil (EVOO).	A 1:1 (v/v) blend of Sesamum indicum oil (AIU-BC-618-1) and Olea europaea oil (AIU-BC-618-2). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by mechanical stirring (1 hr, 40°C).
DIETARY_SUPPLEMENT	50% Psyllium Extract (PE) + 50% Oil Blend (50% SO + 50% EVOO).	50% Plantago ovata mucilage extract (aqueous extraction, 65°C) + 50% AIU-BC-618-3 (SO+EVOO blend). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by sonication (20 min).
DIETARY_SUPPLEMENT	Plantago ovata extract (AIU-BC-618-4) + AIU-BC-618-3	50% Taraxacum officinale root extract (ethanol-water extraction, 70°C) + 50% AIU-BC-618-3 (SO+EVOO blend). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by magnetic stirring (30 min, 50°C).
DIETARY_SUPPLEMENT	50% Herbal Extract (25% PE + 25% DE) + 50% Oil Blend (25% SO + 25% EVOO).	25% Plantago ovata extract (AIU-BC-618-4) + 25% Taraxacum officinale extract (AIU-BC-618-5) + 25% SO (AIU-BC-618-1) + 25% EVOO (AIU-BC-618-2). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by homogenization (45 min, 37°C).
DRUG	Drug: Rowachol® (terpene extract) at 100 mg/mL in dissolving fluid.	25% Plantago ovata extract (AIU-BC-618-4) + 25% Taraxacum officinale extract (AIU-BC-618-5) + 25% SO (AIU-BC-618-1) + 25% EVOO (AIU-BC-618-2). Final concentration: 100 mg/mL in BioIVT bile juice. Prepared by homogenization (45 min, 37°C).

Timeline

• Start date: 2023-12-15
• Primary completion: 2024-05-17
• Completion: 2024-08-28
• First posted: 2025-05-14
• Last updated: 2025-05-18

Locations

1 site across 1 country: Syria