NCT06945146

Genomics Study in CML Patients With Ponatinib Treatment

The Comprehensive Assessment of BCR-ABL1 Gene Expression and Genetic Variations by qRT-PCR and NGS Assays in Chronic Myeloid Leukemia Patients Who Are Treated With Ponatinib (CAP Study)

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 65 (actual)
• Sponsor: Dong-Wook Kim · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

This study will evaluate whether responsiveness and adverse events (AEs) to second-line or later ponatinib treatment are associated with genetic variations as measured by real-time quantitative polymerase chain reaction (qRT-PCR) and next-generation sequencing (NGS) in patients with CML of any stage who failed prior multiple targeted therapies except ponatinib.

Detailed description

Ponatinib treatment will be initiated per usual treatment procedure at 45 mg once daily p.o., which will be gradually decreased to 30 mg and 15 mg according to the predefined criteria based on responsiveness to treatment and AEs in the course of the treatment. A total of 100 subjects will be enrolled within 24 months after the first subject enrollment, and ponatinib treatment will continue for 24 months after the initial dosing of ponatinib in each enrolled subject. Routine ponatinib treatment will continue at the investigator's discretion until disease progression, the occurrence of unacceptable toxicity, subject's withdrawal of consent, or occurrence of any reason for discontinuation specified in the protocol. All molecular analysis samples will be collected, transferred, and managed by the Catholic Leukemia Research Institute, and NGS will be performed. \<Eligibility\> 1. Adults with BCR-ABL1-positive CML 2. Subjects who were resistant or intolerant to prior targeted therapy other than ponatinib and with an indication for ponatinib treatment according to the acceptance criteria by the Ministry of Food and Drug Safety (MFDS) 3. Women of childbearing potential (WOCBP) should have a negative serum or urine pregnancy test (with a sensitivity of at least 25 IU/L or equivalent to HCG) within 24 hours before initiating ponatinib treatment 4. Written informed consent to ponatinib treatment \<Outcome Measures\> 1. Primary endpoint \- 24 months dynamics of BCR-ABL1 gene expression by qRT-PCR 2. Secondary endpoints * Type and frequency of novel genetic variations (mutations, gene expressions, CNV, INDEL, etc.) * Cobll1/GCA/novel gene network identification: functional tests using Western blot/Knock-down assay 3. Safety endpoints : To explore the dynamics of adverse events according to dose changes up to 24 months * Frequency and severity of skin rash, fever, hypertension, pancreatitis and vascular events as common adverse events * Frequency and severity of rare adverse events * Treatment intolerance is defined as recurrence of a Grade ≥3 hematologic AE, or a Grade ≥2 non-hematologic AE requiring permanent discontinuation of ponatinib per protocol despite dose reduction

Conditions

• Chronic Myelogenous Leukemia, BCR-ABL1 Positive

Interventions

Type	Name	Description
DRUG	Ponatinib	Ponatinib treatment will be initiated per usual treatment procedure at 45 mg once daily p.o., which will be gradually decreased to 30 mg and 15 mg according to the predefined criteria based on responsiveness to treatment and AEs in the course of the treatment.

Timeline

• Start date: 2020-09-01
• Primary completion: 2024-08-31
• Completion: 2024-09-25
• First posted: 2025-04-25
• Last updated: 2025-04-25

Locations

1 site across 1 country: South Korea

Regulatory

• FDA-regulated drug study