NCT06906510

Evaluation of Trimethylamine N-oxide (TMAO) Levels in Periodontal Disease

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 48 (actual)
• Sponsor: Ankara Medipol University · Academic / Other
• Sex: All
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

Salivary and serum levels of TMAO and TNF-α can distinguish between individuals with periodontitis and periodontally healthy individuals.

Detailed description

Trimethylamine N-oxide (TMAO), a gut flora-derived metabolite from dietary choline, has emerged as an indicator of atherosclerosis. Circulatory TMAO has been implicated in cardiovascular risks by altering enterohepatic cholesterol and bile acid metabolism, increasing macrophage scavenger receptor expression, and activating nuclear factor kappa B (NF-κB) via pro-inflammatory genes such as interleukin-1 (IL-1). The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α), which plays a crucial role in immune responses and inflammation, has been demonstrated as a crucial participant also during the development of periodontal diseases. The aim of this study is to evaluate difference in salivary and serum levels of TMAO and TNF-α between individuals with periodontitis and periodontally healthy individuals. The study includes two groups: systemically and periodontally healthy control subjects (n= 24), and patients with periodontitis (n=24). Periodontal parameters were recorded. TMAO levels in saliva and serum were determined by liquid chromatography-mass spectrometry (LC-MS/MS), and TNF-α levels were determined by ELISA.

Conditions

• Periodontitis
• Inflammation
• Trimethylamine N-oxide (TMAO)
• TNF-alfa

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Salivary and Serum TMAO Mesurement	Salivary and serum TMAO levels were measured by liquid chromatography-mass spectrometry (LC-MS/MS)
DIAGNOSTIC_TEST	Salivary and Serum TNF-α Mesurement	Salivary and serum TNF-α levels were measured by ELISA

Timeline

• Start date: 2024-01-08
• Primary completion: 2024-08-14
• Completion: 2024-09-23
• First posted: 2025-04-02
• Last updated: 2025-04-02

Locations

1 site across 1 country: Turkey (Türkiye)