NCT06905964

Eccentric Cycling Exercise With Assistive Devices on Mitochondrial Metabolism in T Lymphocyte

Effects of Eccentric Cycling Exercise Training With Assistive Devices on Phenotypes and Mitochondrial Metabolism in T Lymphocytes in Sedentary Men

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 30 (actual)
• Sponsor: Chang Gung Memorial Hospital · Academic / Other
• Sex: Male
• Age: 20 Years – 30 Years
• Healthy volunteers: Accepted

Summary

Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness. Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions. Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.

Conditions

• There Are no Conditions Under Study. Healthy Volunteers

Interventions

Type	Name	Description
BEHAVIORAL	Eccentric cycling exercise (ECC)	Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. After each training session, rest in sitting position for 20 min. Exercise intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.
BEHAVIORAL	Eccentric cycling exercise with assistive devices (ECA)	Performed exercise training 5 days a week for 5 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. After each training session, receive NMES in sitting position for 20 min. Intensity: Firstly, set at 80% Wmax, and progressively increased 5% per week to 120 % Wmax in the last week.

Timeline

• Start date: 2023-02-23
• Primary completion: 2024-02-23
• Completion: 2024-12-31
• First posted: 2025-04-02
• Last updated: 2025-04-02

Locations

1 site across 1 country: Taiwan