NCT06834815

Investigating the Bactericidal Effect of Probiotics on Chromogenic Bacteria in Children An In Vivo and In Vitro Study

Effect of Probiotics on Chromogenic Bacteria and Extrinsic Stains in Children

• Status: Completed
• Phase: Phase 4
• Study type: Interventional
• Enrollment: 9 (actual)
• Sponsor: Ain Shams University · Academic / Other
• Sex: All
• Age: 6 Years – 14 Years
• Healthy volunteers: Accepted

Summary

To investigate the bactericidal effect of probiotics (Streptococcus salivarius M18 and Lactobacillus reuteri) on two types of bacteria (Aggregatibacter actinomycetemcomitans and Actinomyces naeslundii) causing black stains of teeth in vitro.

Detailed description

In vitro: An unstimulated salivary sample will be taken from patients having black stains. The two types of bacteria of each condition will be cultured, amplified, and measured in the laboratory from the saliva of these patients. After that, one type of probiotics will be applied to these bacteria separately and the same bacterial measurement will be done to evaluate the bactericidal effect of the probiotics on these bacteria, aiming to find the minimal inhibitory concentration of the probiotics.

Conditions

• Black Stains

Interventions

Type	Name	Description
DRUG	Probiotic with Prebiotic	After assigning the patients , all patients will submit an unstimulated salivary sample of 1ml will in sealed test tubes then stored in a deep freezer until microbiological evaluation, then they'll receive an oral prophylaxis session. * The patients will receive probiotics for 14 days given to them in a sealed box. * All patients will be followed up after 14 days and another salivary sample will be collected and sent for microbiological assessment.

Timeline

• Start date: 2024-11-05
• Primary completion: 2025-02-20
• Completion: 2025-03-01
• First posted: 2025-02-19
• Last updated: 2025-04-16

Locations

1 site across 1 country: Egypt