NCT06820944

Theobromine From Cocoa and Cardiovascular Risk Factors

Theobromine Consumption From Cocoa and Cardiovascular Risk Factors in Humans: Evidence for Health Claims

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 53 (actual)
• Sponsor: Chiang Mai University · Academic / Other
• Sex: Male
• Age: 35 Years – 70 Years
• Healthy volunteers: Not accepted

Summary

The goal of this clinical trial is to examine whether consumption of dark chocolate affects blood pressure and a cardiovascular risk factor called trimethylamine N-oxide in Thai male participants with hypertension. The main questions it aims to answer are: Can consumption of dark chocolate lower blood pressure? Can consumption of dark chocolate lower blood trimethylamine N-oxide? Researchers will compare dark chocolate to white chocolate to see if the effects are due to theobromine (a key bioactive compound in dark chocolate). Participants will consume either 100 g 72% dark chocolate bar or 80 g white chocolate bar daily for 14 days, rest for 7 days, and then switch to the other type of chocolate.

Detailed description

This is a randomized cross-over trial conducted in Chiang Mai, Thailand. The goal of this clinical trial is to examine whether consumption of dark chocolate affects cardiovascular risk factors, with the primary outcomes being blood pressure and a cardiovascular risk factor called trimethylamine N-oxide. Participants are Thai males aged 35-70 years with hypertension who live in the Chiang Mai area. 42 Participants will consume either 100 g 72% dark chocolate bar (\~2.5 servings, providing \~900 mg theobromine) or 80 g white chocolate bar (\~2 servings, providing 0 mg theobromine) daily for 14 days. After a 7-day wash-out period, the participant will repeat the protocol with the other intervention.

Conditions

• Hypertension

Interventions

Type	Name	Description
OTHER	dark chocolate	Consumption of 100 g/day of 72% dark chocolate bar for 14 days consecutively
OTHER	white chocolate	Consumption of 80 g/day of white chocolate bar for 14 days consecutively

Timeline

• Start date: 2025-02-15
• Primary completion: 2025-04-30
• Completion: 2025-04-30
• First posted: 2025-02-11
• Last updated: 2026-03-18

Locations

1 site across 1 country: Thailand