NCT06801652

The Influence of Sperm Morphology and Functional Indices on Blastocyst Development in Assisted Reproduction

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 120 (actual)
• Sponsor: Calla IVF Center · Academic / Other
• Sex: All
• Age: 21 Years – 40 Years
• Healthy volunteers: —

Summary

Samples from male partners of 120 infertile couples undergoing IVF were analyzed. Sperm morphology was assessed using the SpermBlue staining method, while TZI, MAI, and SDI were calculated. Embryo development was monitored until day 5 or 6. Statistical analyses included regression models and correlation coefficients to evaluate the predictive value of sperm indices on blastocyst development.

Detailed description

For spermatozoa selection we used ZyMot multi in all patients undergoing IVF. After intracytoplasmatic injection of spermatozoa we asses the blastocyst grading in day 5. NEQSI score meaning numerical embryonic quality score index was used for statistical purpose. The spermatozoa morphology indexes were: TZI (teratozoospermia index), MAI (multiple anomalies index), SDI (sperm deformity index) and analyzed with CASA analyzer. All these indexes were assessed in patients and correlated in all groups: patients that had a positive pregnancy test, patients that had a negative pregnancy rate, patients that had no blastocyst in day 5 and patients that had all aneuploid embyos.

Conditions

• Spermatozoa, Abnormal
• in Vitro Fertilization
• Blastocyst
• Aneuploidy

Interventions

Type	Name	Description
OTHER	Sperm morphology indexes	We analyzed all sperm morphology indexes in all groups.

Timeline

• Start date: 2023-10-21
• Primary completion: 2024-10-23
• Completion: 2024-11-10
• First posted: 2025-01-30
• Last updated: 2025-01-30

Locations

1 site across 1 country: Romania