NCT06735625

Pilot Study "AHSP as a Biomarker of Sickle Cell Disease in a Population of Adults and Children"

• Status: Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 100 (estimated)
• Sponsor: Centre Hospitalier de Saint-Denis · Academic / Other
• Sex: All
• Age: 3 Years
• Healthy volunteers: Not accepted

Summary

Evaluation of AHSP concentration in total blood as a biomarker in adult and pediatric sickle cell patients

Detailed description

Research involving non-interventional humans. Selection and inclusion of patients by CHSD investigators. Collection of clinical data by investigators and CHSD URC staff. Biochemical and hematological measurements by the CHSD medical biology laboratory for "care" samples Pseudonymization of 4 mL "research" samples by the URC then fractionation of the samples by 1 mL, and storage at -80°C within the medical biology laboratory of the CHSD. Transport of samples at -80°C to team 1 of Dr Baudin-Creuza (Créteil). Preparation of genomic DNA from a 1 mL fraction then α and β globin genotyping by Dr Pissard. Measurement of the AHSP concentration from the other fractions. Comparison of the AHSP concentration according to the group of subjects, and with the different parameters, then correlation analysis

Conditions

• in Relation to Sickle Cell Disease

Interventions

Type	Name	Description
BIOLOGICAL	Measurement of AHSP concentration	Pseudonymization and identification of research samples EDTA tube of 4 mL for patients over 15 and a half years old, and 2 EDTA tubes of 2 mL for children aged 3 to 15 and a half years, totaling 4 mL) by Team 4 according to the procedure in force at the URC. The 4 mL research sample will then be divided into 1 mL fractions by a technician in the laboratory and stored at -80°C in the medical biology laboratory.

Timeline

• Start date: 2025-03-06
• Primary completion: 2026-03-20
• Completion: 2027-01-20
• First posted: 2024-12-16
• Last updated: 2025-04-02

Locations

1 site across 1 country: France