NCT06714149

Impacts of Nitrogen Deposition in the Natural Environment on Pollen Allergy in Belgium

• Status: Recruiting
• Phase: N/A
• Study type: Interventional
• Enrollment: 200 (estimated)
• Sponsor: prof. dr. Rik Schrijvers · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

The investigators want to study the effect of environmental nitrogen enrichment on the allergy burden in pollen-sensitized subjects.

Detailed description

The investigators want to study allergen-specific IgE reactivity from a cross-sectional sample of pollen-allergic patients (and negative controls) against environmental and experimental pollen samples. Following aspects will be compared: * To study the basophil degranulation with different amounts of pollen on fresh patient-derived basophils, to determine the concentration of pollen needed to degranulate 5% (EC5), and 50% (EC50) of basophils. * To study he overall intensity (quantitative), as well as IgE-mediated hyper- or hyporecognition of different fragments in the pollen samples (qualitative) in a subset of patients. * To associate allergic rhinoconjunctivitis and food-pollen syndrome related symptoms with reactivity to different pollen samples.

Conditions

• Pollen Allergy

Interventions

Type	Name	Description
DIAGNOSTIC_TEST	Immunoreactivity testing	Blood samples will be collected and used to determine the intensity of the allergenicity of the subject toward different types of pollen. This will be done by the detection of sIgE antibodies to the pollen extracts and basophil activation testing (BAT) with pollen extracts.
DIAGNOSTIC_TEST	Immunoreactivity testing (negative control)	Blood samples will be collected and used to determine the background activity of our immunoreactivity tests. The investigators will use samples from negative controls to demonstrate that non-allergic individuals do not react to our assays, confirming that our pollen extracts have no background reactivity. This will be done by the detection of sIgE antibodies to the pollen extracts and basophil activation testing (BAT) with pollen extracts.

Timeline

• Start date: 2022-06-29
• Primary completion: 2025-02-01
• Completion: 2025-04-01
• First posted: 2024-12-03
• Last updated: 2024-12-03

Locations

1 site across 1 country: Belgium