NCT06588049

Nitrate Mouth Rinse and the Oral Microbiome

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 21 (actual)
• Sponsor: Dalhousie University · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

The study is aimed to determine if changes to the oral microbiome occur after the use of a nitrate rich mouth rinse. Participants with be asked to use either a controlled (water) mouthrinse once daily for 2 weeks while maintaining normal oral hygiene practices, or use the nitrate rich mouthrinse once daily for 2 weeks. Primary Outcome measures: -changes in the composition of the oral microbiome Secondary Outcome measures * Blood Pressure * Salivary Nitric Oxide Levels

Detailed description

Nitrate is found in high concentrations in beet root, and as such we will use a beet root based product as a daily mouth rinse (Swish and Spit) as a part of the participants daily oral hygiene regimen and collect the participants biofilm before and after 2 weeks of regular use. We will then analyze the data using 16s rRNA sequencing to evaluate the changes in alpha diversity, beta diversity and the absolute and relative differential abundance of nitrate reducing bacteria in the oral microbiome. We will also collect salivary samples to evaluate NO levels, as well as measure blood pressure pre and post treatment. After this study's completion, we expect to have gained new knowledge on nitrate's influence on the oral microbiome's composition. Gaining this knowledge will allow better public understanding of the effects oral hygiene products have on their oral and systemic health. In addition, the knowledge gained could encourage further investigations into how to better formulate oral hygiene products for improved health and performance.

Conditions

• Nitric Oxide
• Oral Microbiome

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Beet Root	Beet Root powder used as a mouth rinse in suspension with water
OTHER	Water	Water used as a mouth rinse

Timeline

• Start date: 2024-08-14
• Primary completion: 2025-05-16
• Completion: 2025-05-16
• First posted: 2024-09-19
• Last updated: 2025-09-02

Locations

1 site across 1 country: Canada