NCT06576804

Eccentric Cycling Exercise on Mitochondrial Function of Lymphocyte

Effects of Concentric and Eccentric Exercise Regimens on Bioenergetic Efficiency of Lymphocytes in Sedentary Males

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 33 (actual)
• Sponsor: Chang Gung Memorial Hospital · Academic / Other
• Sex: Male
• Age: 20 Years – 30 Years
• Healthy volunteers: Accepted

Summary

Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established. Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

Detailed description

Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET). Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions. Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.

Conditions

• Eccentric Exercise Training

Interventions

Type	Name	Description
BEHAVIORAL	Eccentric cycling training	Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week
BEHAVIORAL	Concentric cycling training	Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Timeline

• Start date: 2021-01-01
• Primary completion: 2022-01-01
• Completion: 2022-08-01
• First posted: 2024-08-29
• Last updated: 2024-08-29

Locations

1 site across 1 country: Taiwan