NCT06560216

Cryopreservation Medium With Apigenin on Post-thaw Human Sperm

Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 57 (actual)
• Sponsor: Chulalongkorn University · Academic / Other
• Sex: Male
• Age: 18 Years – 45 Years
• Healthy volunteers: Accepted

Summary

The goal of this clinical trial is to compare the effect of apigenin supplementation in freezing medium with no apigenin supplementation in freezing medium on post-thaw human sperm motility. It will also evaluate the sperm viability, total antioxidant capacity, and oxidative stress markers. The main questions it aims to answer are: * Does apigenin supplementation in freezing medium improve the post-thaw sperm motility? * Does apigenin supplementation in freezing medium improve the post-thaw sperm viability, total antioxidant capacity, and oxidative stress?

Detailed description

All normal semen samples from participants who give written informed consent and meet the eligibility requirements will be included in the study. Prepared semen samples were divided into 3 groups. 1. Pre-freezing sperm suspension 2. Frozen-thawed control without apigenin supplementation in freezing medium 3. Frozen-thawed sperm suspension with apigenin 0.2 mM supplementation in freezing medium Baseline sperm motility will be measured in pre-freezing sperm suspension. The last 2 groups will be stored in liquid nitrogen for at least one week. After thawing, the sperm suspensions will be evaluated for motility, viability, total antioxidant capacity, and oxidative stress markers.

Conditions

• Healthy

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Apigenin	0.2 mM in freezing medium

Timeline

• Start date: 2024-10-28
• Primary completion: 2025-02-12
• Completion: 2025-03-03
• First posted: 2024-08-19
• Last updated: 2025-03-18

Locations

2 sites across 1 country: Thailand